Following are the pastors who have served over the years at St. John's:
| 1880-1882 | F.W. Spindler |
| 1882 | Rev. Junkers* |
| 1882-1883 | Rev. Niedergesaesz* |
| 1883-1886 | Jacob Maierle |
| 1886-1892 | J. Ernst Birkner |
| 1892-1897 | Bernhard Vogelsang |
| 1897-1903 | Henry Kunz |
| 1903-1924 | Conrad Bizer |
| 1924-1929 | Rudolph Zielinski |
| 1930-1937 | A.P.C. Fredric Bemberg |
| 1937-1940 | Armin A. Suedmeyer |
| 1941-1950 | Emil Roth |
| 1950-1951 | Paul Franzmeier* |
| 1951-1967 | Edmond De Buhr |
| 1967 | James Ford* |
| 1968-1970 | Bert Downey |
| 1970-1971 | Calvin Helming* |
| 1971-1975 | C. Kent Wever |
| 1975-1976 | Francis Jones* |
| 1976-1979 | James Parker |
| 1979-1980 | Edgar Adair* |
| 1980-1987 | Milton Kading |
| 1987 | Diane Shelley* |
| 1987-1988 | Lane Zaffke* |
| 1988-1990 | Doug & Lou Gordon-Denniston |
| 1990-1991 | Jan Bodin** |
| 1991-1997 | Larry & Laura Odegard |
| 1997 | Bill Reese* | 1997-1999 | Glen Holland** |
| 1999-date | Paul Burgess |
* = supply pastor
** = interim pastor
Since 1954, St. John's has been yoked with Mt. Hope Presbyterian Church.